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Related Experiment Videos

Endothelial barrier function under laminar fluid shear stress.

J Seebach1, P Dieterich, F Luo

  • 1Institut für Biochemie, WWU-Münster, Germany.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|January 5, 2001
PubMed
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Increased shear stress alters endothelial permeability and cell behavior, affecting transendothelial electrical resistance (TER) and junction protein organization without compromising monolayer integrity.

Area of Science:

  • Cardiovascular Biology
  • Cellular Biophysics
  • Endothelial Cell Research

Background:

  • Endothelial permeability is crucial for vascular health and disease.
  • Shear stress is a key hemodynamic factor influencing endothelial cells.
  • Understanding shear stress effects is vital for venous grafting and vascular disease pathogenesis.

Purpose of the Study:

  • To investigate the online effects of laminar shear stress on transendothelial electrical resistance (TER).
  • To analyze shear stress-induced changes in endothelial cell morphodynamics and junction protein organization.
  • To determine the impact of shear stress on endothelial monolayer integrity.

Main Methods:

  • Development of a novel impedance spectroscopy setup for online TER measurement.

Related Experiment Videos

  • Application of pure laminar shear stress to confluent porcine pulmonary artery endothelial cell cultures.
  • Analysis of cell motility, shape changes (morphodynamics), and catenin distribution under varying shear stress levels.

    • Main Results:

    • Shear stress (2-50 dyn/cm2) transiently increased TER by 2-15% within 15 minutes, reducing cell motility.
    • Sustained shear stress led to a subsequent decrease in TER and accelerated cell shape changes.
    • TER recovered to baseline levels within hours, accompanied by decelerated shape changes.
    • Heterogeneous distribution and plasma membrane colocalization of alpha-, beta-, and gamma-catenin were observed.
    • No intercellular gap formation was detected, even at peak shear stress levels.

    Conclusions:

    • Endothelial monolayers exhibit shear stress-dependent regulation of permeability and junction protein reorganization.
    • Increased laminar shear stress modulates endothelial cell morphodynamics and barrier function.
    • Endothelial monolayer integrity is maintained despite significant shear stress exposure and associated protein rearrangement.