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Updated: Jul 10, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
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Determination of paroxetine levels in human plasma using gas chromatography with electron-capture detection
C T Lai1, E S Gordon, S H Kennedy
1Department of Psychiatry, Mackenzie Health Sciences Centre, University of Alberta, Edmonton, Canada. ctlai@gpu.srv.ualberta.ca
Abstract:
A simple, rapid and sensitive procedure using gas chromatography with electron-capture detection to measure paroxetine levels in human plasma has been developed. The analyte was extracted from plasma with ethyl acetate after basification of the plasma and then derivatized with heptafluorobutyric anhydride before gas chromatographic separation. The calibration curves were linear, with typical r2 values >0.99. The assay was highly reproducible and gave peaks with excellent chromatographic properties.
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