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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
CD11b expression identifies CD8+CD28+ T lymphocytes with phenotype and function of both naive/memory and effector
S Fiorentini1, S Licenziati, G Alessandri
1Institute of Microbiology and Department of Infectious Diseases, University of Brescia Medical School, Brescia, Italy.
Journal of Immunology (Baltimore, Md. : 1950)
|January 6, 2001
Summary
A novel CD8(+)CD28(+)CD11b(+) T cell subset, found in healthy individuals and expanding during viral infections, exhibits intermediate immune functions. This discovery helps distinguish memory and effector T cells.
Area of Science:
- Immunology
- Cell Biology
- T cell differentiation
Background:
- A previously unidentified T cell subset, CD8(+)CD28(+)CD11b(+), exists in healthy individuals.
- This subset expands during primary viral infections, suggesting a role in immune responses.
Purpose of the Study:
- To characterize the phenotype and function of the novel CD8(+)CD28(+)CD11b(+) T cell subset.
- To investigate its potential role as an intermediate in T cell differentiation.
Main Methods:
- Phenotypic analysis of T cell subsets using flow cytometry.
- Functional assays measuring cytokine production (IFN-gamma, IL-2), cytotoxicity, and proliferation.
- In vitro generation of the subset from naive T cells.
Main Results:
- CD8(+)CD28(+)CD11b(+) T cells exhibit dual functions: cytolytic activity and IFN-gamma production (effector-like), alongside IL-2 production and proliferation capacity (memory-like).
- These cells can migrate and cross endothelial barriers.
- The subset can be generated in vitro from naive CD8(+)CD28(+)CD11b(-) T cells upon activation and memory phenotype acquisition.
Conclusions:
- The CD8(+)CD28(+)CD11b(+) T cell subset represents an intermediate stage in CD8(+) T cell differentiation.
- CD11b expression serves as a marker to differentiate between memory and effector phenotypes within the human CD8(+)CD28(+) T cell population.
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