Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme engineering: rational redesign versus directed evolution.

R Chen1

  • 1Dept of Biochemistry, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada. ridong.chen@monsanto.com

Trends in Biotechnology
|January 9, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A reversed phase HPLC method with pre-column derivatization to determine propafenone enantiomers in human plasma].

Yao xue xue bao = Acta pharmaceutica Sinica·2002
Same author

[Study on the polyoxygenated cyclohexenes from Uvaria boniana].

Yao xue xue bao = Acta pharmaceutica Sinica·2002
Same author

[Systemic side effects of long-term treatment with low dose inhaled corticosteroids in children with asthma].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2002
Same author

[Effects of different cryoprotectants on biological properties of hematopoietic cells derived from cord blood].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2002
Same author

[Study of telomerase activity and its related genes expression in leukemia cells].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2002
Same author

[Effects of selective RAR or/and RXR retinoids on the proliferation and differentiation of NB4 cells and their mechanisms].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2002
Same journal

Engineering a capture-bioremediate-release microbial biofilm for simultaneous bioremediation of microplastics and adsorbed heavy metals.

Trends in biotechnology·2026
Same journal

Engineered bacterial biofilms for biotechnological applications.

Trends in biotechnology·2026
Same journal

Multiscale and programmable engineering of edible mushroom mycelium-based materials.

Trends in biotechnology·2026
Same journal

Transporter engineering in microbial cell factories.

Trends in biotechnology·2026
Same journal

Random integration and high-throughput screening forging robust microbial cell factories.

Trends in biotechnology·2026
Same journal

Reprogrammable bacterial nanosyringes to deliver RNA and gene editors.

Trends in biotechnology·2026
See all related articles

Enzyme engineering is rapidly advancing, offering new possibilities for enzyme applications. This review briefly discusses the perspectives of rational redesign and directed evolution strategies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Enzyme engineering is experiencing significant advancements.
  • This field aims to create enzymes with enhanced physical and catalytic properties.
  • Applications of engineered enzymes are expanding rapidly.

Purpose of the Study:

  • To discuss the perspectives of enzyme engineering strategies.
  • To provide a brief overview of rational redesign and directed evolution.

Main Methods:

  • Rational redesign of enzymes.
  • Directed evolution of enzymes.

Main Results:

  • Both rational redesign and directed evolution have achieved considerable success.

Related Experiment Videos

  • Each strategy presents unique limitations.
  • Further development is expected for both approaches.

    • Conclusions:

    • Enzyme engineering holds immense potential for future applications.
    • Understanding the strengths and weaknesses of current strategies is crucial for progress.