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Updated: Oct 9, 2026

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes
Published on: December 19, 2025
Peptide-based targeting of fluorophores to peroxisomes in living cells
E H Pap1, T B Dansen, K W Wirtz
1Biochemistry of Lipids Dept, Utrecht University, PO Box 80.054, 3508 TB, Utrecht, The Netherlands.
Abstract:
Peptides carrying different fluorophores can be designed to incorporate spontaneously into living cells when added to the medium. By incorporating the peroxisome-targeting sequence PTS1, the peptide is recognized by the protein-import machinery of peroxisomes and, as a result, can accumulate in these organelles. Depending on the cell type, an inhibitor of the multidrug-resistance protein might be required to ensure strong accumulation. In this update, we discuss the potential of these peptide-linked fluorophores in solving issues related to organelle function and dynamics.
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