[Feasibility of the use of elastographic techniques with ultrasound in the diferentiation of different tissues]

To investigate the feasibility of distinguishing different tissues by detecting their different elastic properties when mechanically stimulated with an ultrasound wide beam.

A tissue-mimicking material phantom was placed in a water filled box. Two devices (Teuco Hydrosonic) emitting two US beams (3.2 MHz) were placed on two opposite walls of the box. The US beams had a circular diameter of about 10 cm; their intensity could be set by varying the power voltage applied to the device from 0 to 12 V. The US beams caused a mechanical stimulation of both the phantom and the water surrounding it. An ATL HDI 5000 scanner was used in order to acquire tissue Color Doppler images using the fundamental frequency (Tissue Doppler Imaging, TDI), and the second harmonic (Harmonic Tissue Doppler Imaging, HTDI). The images allowed to visualize, using a color scale, the vibrational status of both the phantom and the water surrounding it. Both fundamental and second harmonic images were taken using different power values supplied to the stimulation device, with the voltage ranging from 0 to 12 V. In order to quantify the vibrational status of the water and of the tissue-mimicking phantom two Regions Of Interest (ROI) were taken on the images. A parameter describing the "color amount" in each ROI and defined by an appropriate mathematical function was proposed.

Differences between the values of the "color amount" parameter in the phantom and in water were statistically significant both at the fundamental frequency and at second harmonic imaging, and for each positive value of the voltage of the power supplied to the device for the stimulation. Also the differences between the parameter "color amount" at the fundamental frequency and second harmonic imaging were statistically significant; the differences were found for both water and phantom and for any positive voltage. The correlation between the intensity of the stimulation (voltage) and the "color amount" parameter is statistically significant with the second harmonic technique in the phantom, nearly significant with the second harmonic technique in water and not significant in the other cases.

It is possible to try to characterize a tissue by analyzing its elastic properties when an ultrasound wide beam mechanically stimulates it. The most promising technique consists in acquiring images using Harmonic Tissue Doppler Imaging, (HTDI) and measuring the vibrational status by using the "color amount" parameter proposed.