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Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex.

I M Cheeseman¹, M Enquist-Newman, T Müller-Reichert

¹Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.

The Journal of Cell Biology

|January 10, 2001

Summary

The Duo1p/Dam1p complex in Saccharomyces cerevisiae maintains mitotic spindle integrity and links kinetochores to the spindle, preventing chromosome missegregation.

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Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Duo1p and Dam1p are known spindle proteins in budding yeast.
Previous studies identified Duo1p and Dam1p as spindle proteins.

Purpose of the Study:

To elucidate the functions and interactions of Duo1p and Dam1p.
To investigate the role of the Duo1p/Dam1p complex in mitosis.

Main Methods:

Analysis of duo1 and dam1 alleles.
Genetic interaction studies.
Co-immunoprecipitation assays.
Immunofluorescence and electron microscopy.
Kinetochore localization studies.

Main Results:

Duo1p and Dam1p form a complex with shared functions in vivo.

Related Experiment Videos

The Duo1p/Dam1p complex is essential for maintaining metaphase and anaphase spindle integrity.

Mutants exhibit spindle defects, chromosome missegregation, and premature anaphase events.

Duo1p and Dam1p localize to kinetochores, suggesting a role in kinetochore-spindle linkage.

Conclusions:

Duo1p and Dam1p function as a complex essential for mitotic spindle integrity.
The Duo1p/Dam1p complex plays a novel role in kinetochore function.
This complex may act as a crucial link between kinetochores and the mitotic spindle.