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Related Experiment Videos

GDNF is a chemoattractant for enteric neural cells.

H M Young1, C J Hearn, P G Farlie

  • 1Department of Anatomy & Cell Biology, University of Melbourne, Victoria, 3010, Australia.

Developmental Biology
|January 11, 2001
PubMed
Summary

Glial cell line-derived neurotrophic factor (GDNF) influences embryonic mouse gut development. GDNF attracts neural crest cells, promoting their migration within the gut and preventing them from migrating outside.

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Area of Science:

  • Developmental Biology
  • Neuroscience
  • Gastroenterology

Background:

  • Glial cell line-derived neurotrophic factor (GDNF) is crucial for neural development.
  • Understanding GDNF's role in enteric nervous system formation is vital for developmental studies.

Purpose of the Study:

  • To investigate the spatiotemporal expression of GDNF mRNA in embryonic mouse gut.
  • To determine the effect of exogenous GDNF on the migration and differentiation of enteric neural crest-derived cells.
  • To assess GDNF's chemoattractant properties for neural crest cells in various embryonic tissues.

Main Methods:

  • In situ hybridization to detect GDNF mRNA expression in embryonic mouse gut.
  • Catenary organ culture of embryonic mouse hindgut explants with and without GDNF.

Related Experiment Videos

  • Chemotaxis assays using GDNF-impregnated agarose beads and explants from different embryonic tissues.

    • Main Results:

    • GDNF mRNA expression increased significantly in the mid- and hindgut mesenchyme after embryonic day 11.5.
    • Exogenous GDNF reduced neuron development within hindgut explants but increased migration of neural crest cells outside the explant.
    • Neural crest cells and neurites from the esophagus and midgut exhibited chemotaxis towards GDNF, while other neural tissues showed minimal response.

    Conclusions:

    • GDNF acts as a chemoattractant for enteric neural crest-derived cells, guiding their migration within the gastrointestinal tract.
    • GDNF may prevent neural crest cells from migrating into surrounding tissues like the mesentery.
    • GDNF promotes directed axon outgrowth and plays a significant role in enteric nervous system development.