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cDNA micro array identification of a gene differentially expressed in adenovirus type 5- versus type 12-transformed
A C Vertegaal1, H B Kuiperij, T van Laar
1Laboratory for Molecular Carcinogenesis, MGC-Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands.
Abstract:
Proteins encoded by non-oncogenic adenovirus type 5 and oncogenic adenovirus type 12 differentially affect expression of a number of cellular genes. We have used cDNA micro array analysis to identify a cellular gene that is expressed in Ad12- but not in Ad5-transformed cells. This cellular gene was found to be the gene encoding follistatin-related protein, a TGF-beta inducible gene. Consistently, a constitutive factor binding to Smad binding elements was found in adenovirus type 12-transformed cells.
Insights
Oncogenic adenovirus type 12 (Ad12) alters cellular gene expression, unlike non-oncogenic adenovirus type 5 (Ad5). Researchers identified follistatin-related protein as a key gene upregulated in Ad12-transformed cells, linked to constitutive Smad binding.
Area of Science:
- Molecular biology
- Virology
- Cancer research
Background:
- Adenovirus type 5 (Ad5) and adenovirus type 12 (Ad12) are distinct viral pathogens.
- Differential expression of cellular genes is observed between Ad5 and Ad12 infections.
- Understanding these molecular differences is crucial for cancer research.
Purpose of the Study:
- To identify cellular genes differentially expressed in Ad12-transformed cells compared to Ad5-transformed cells.
- To investigate the role of follistatin-related protein in Ad12 oncogenesis.
- To explore the mechanism of altered gene expression in Ad12-transformed cells.
Main Methods:
- Utilized cDNA microarray analysis to compare gene expression profiles.
- Investigated the expression of follistatin-related protein in Ad5 and Ad12 transformed cells.
- Assessed the binding of constitutive factors to Smad binding elements.
Main Results:
- Identified follistatin-related protein as a gene specifically expressed in Ad12-transformed cells, but not in Ad5-transformed cells.
- Confirmed follistatin-related protein as a transforming growth factor-beta (TGF-β) inducible gene.
- Detected constitutive factor binding to Smad binding elements in Ad12-transformed cells.
Conclusions:
- Adenovirus type 12 significantly alters cellular gene expression, upregulating follistatin-related protein.
- The upregulation of follistatin-related protein in Ad12-transformed cells may be linked to TGF-β signaling pathways.
- Constitutive binding of factors to Smad binding elements in Ad12-transformed cells suggests a mechanism for altered gene regulation.