Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Beta-amyloid precursor polypeptide in SAMP8 mice affects learning and memory.

J E Morley1, V B Kumar, A E Bernardo

  • 1Geriatric Research, Education and Clinical Center, St. Louis Department of Veterans Affairs Medical Center, 63104, St. Louis, MO, USA. morley@slu.edu

Peptides
|January 11, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of metformin on nitric oxide synthase in genetically obese (ob/ob) mice.

Life sciences·2001
Same author

Mungbean [Vigna radiata (L.) Wilczek] globulins: purification and characterization.

Journal of agricultural and food chemistry·2001
Same author

Peripheral steroid sulfatase inhibition potentiates improvement of memory retention for hippocampally administered dehydroepiandrosterone sulfate but not pregnenolone sulfate.

Psychoneuroendocrinology·1999
Same author

Septo-hippocampal drug interactions in post-trial memory processing.

Brain research·1999
Same author

Changes in membrane fatty acids and delta-9 desaturase in senescence accelerated (SAMP8) mouse hippocampus with aging.

Life sciences·1999
Same author

Effect of age on calcium-dependent proteins in hippocampus of senescence-accelerated mice.

Brain research·1999

Aging mice show memory loss linked to amyloid precursor protein (APP) and amyloid-beta (Abeta) buildup in the brain. Targeting Abeta protein reversed these cognitive deficits, suggesting a key role in age-related memory impairment.

Area of Science:

  • Neuroscience
  • Aging Research
  • Molecular Biology

Background:

  • Senescence accelerated mice (SAMP8) exhibit age-related memory and learning deficits.
  • Increased expression of amyloid precursor protein (APP) and its mRNA is observed in the hippocampus with age in these mice.

Purpose of the Study:

  • To investigate the relationship between amyloid precursor protein (APP) expression, amyloid-beta (Abeta) plaque formation, and cognitive decline in aging mice.
  • To determine if targeting Abeta protein can alleviate age-related memory deficits.

Main Methods:

  • Utilized senescence accelerated mice (SAMP8) as a model for aging.
  • Quantified amyloid precursor protein (APP) and its mRNA expression in the hippocampus.
  • Employed immunocytochemistry to assess APP expression and Abeta plaque formation.

Related Experiment Videos

  • Administered antibodies to Abeta protein into the cerebral ventricles of the mice.
  • Assessed cognitive function using acquisition and retention tests.

    • Main Results:

    • Confirmed age-related increases in APP and its mRNA in the hippocampus of SAMP8 mice.
    • Immunocytochemical data indicated a correlation between APP expression levels and Abeta plaque generation.
    • Administration of anti-Abeta antibody into the cerebral ventricles significantly alleviated deficits in acquisition and retention test performance.
    • Demonstrated a reversal of cognitive deficits following Abeta protein targeting.

    Conclusions:

    • Established a direct link between elevated APP and Abeta protein expression and age-related learning and memory loss in SAMP8 mice.
    • Suggests that Abeta protein accumulation is a critical factor contributing to cognitive impairment during aging.
    • Highlights the therapeutic potential of targeting Abeta protein to counteract age-related cognitive decline.