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Related Experiment Videos

A moderate decrease in temperature inhibits the calcium signaling mechanism(s) of the regulatory volume decrease in

M M Souza1, R T Boyle

  • 1Department of Cell Biology, Duke University Medical Center, Durham, NC, USA. mmsouza@uel.br

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|January 11, 2001
PubMed
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Room temperature impairs chick cardiomyocyte volume regulation. Delayed calcium signaling and persistent high intracellular calcium prevent regulatory volume decrease (RVD), potentially causing cell injury.

Area of Science:

  • Cardiovascular Physiology
  • Cellular Biology
  • Biophysics

Background:

  • Chick cardiomyocytes exhibit regulatory volume decrease (RVD) through taurine efflux.
  • This RVD is mediated by calcium (Ca2+) influx via stretch-activated channels at physiological temperatures.
  • The effect of reduced temperatures on this process is not well understood.

Purpose of the Study:

  • To investigate the impact of room temperature (26°C) on the RVD of chick cardiomyocytes.
  • To compare the cellular response to hyposmotic swelling at 37°C versus 26°C.
  • To elucidate the role of intracellular Ca2+ transients in temperature-dependent RVD.

Main Methods:

  • Isolation and culture of cardiomyocytes from 11-day-old chick embryos.
  • Exposure to hyposmotic solution (180 mOsm/kg H2O) at 37°C and 26°C.

Related Experiment Videos

  • Measurement of cell volume changes and intracellular free Ca2+ using fura-2.

    • Main Results:

    • Cells at 26°C showed greater swelling (peak relative volume 1.74 ± 0.06) and failed to recover compared to 37°C cells (peak 1.61 ± 0.03, recovery to 1.22 ± 0.04).
    • Transient intracellular Ca2+ increases were observed at both temperatures, but were significantly larger (115.2 ± 42.8% vs. 29.1 ± 4.5%) and delayed (109 ± 34 s vs. 38 ± 9 s) at 26°C.
    • Intracellular Ca2+ was not cleared to baseline levels at 26°C, unlike at 37°C.

    Conclusions:

    • Room temperature inhibits the RVD in chick cardiomyocytes.
    • Delayed and excessive Ca2+ transients at lower temperatures disrupt the signaling cascade necessary for RVD.
    • Persistent high intracellular Ca2+ at room temperature may lead to unregulated enzyme activity, irreversible injury, and cell death.