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[Inhibitory effect of nicardipine on fibroblast proliferation mechanisms]
I Pinilla1, L B Izaguirre, F J Gonzalvo
1Servicio de Oftalmología, Hospital Universitario Miguel Servet, Zaragoza, Unidad Mixta de Investigación, Universidad de Zaragoza, España.
Purpose:
To assess the effect of nicardipine (NCP) on fibroblast migration and proliferation, and its cellular toxicity.
Methods:
In vitro wound repair was assessed in confluent fibroblast monolayer. Mechanical round wounds were performed in the monolayers and the cultures were incubated in fresh media plus NCP. The cell-free area was monitored after 0, 18, 24 and 48 hours. Groups of treatment: Group 1, Sham. Group 2, NCP 10(-4)M in the media. Group 3, NCP 7.5x10(-5)M. Group 4, NCP 5x10(-5)M. Group 5, NCP 2.5x10(-5)M. Group 6, NCP 10(-5)M. Group 7, NCP 10(-6)M. Group 8, NCP 10(-7)M. Group 9, NCP 10(-3)M. Each experiment consisted of three tests that were repeated four times.
Results:
The fibroblast migration and proliferation was inhibited at 5x10(-5)M or higher doses. The proliferation after 48 hours with NCP 2.5x10(-5)M was statistically inferior to the control group and groups 7, 8, and 9. NCP 5x10(-5)M or higher doses showed cellular atypia and cell death.
Conclusions:
NCP effectively inhibits fibroblastic wound repair process at doses 2.5x10(-5)M and shows toxicity at doses over 5x10(-5)M.