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Adherence of Porphyromonas gingivalis to gingival epithelial cells: modulation of bacterial protein expression
G Agnani1, S Tricot-Doleux, L Du
1Equipe de Biologie Buccale UPRES-EA 1256, Université de Rennes I, 2 place Pasteur, F-35000 Rennes, France.
Oral Microbiology and Immunology
|January 13, 2001
Abstract:
The protein profiles of Porphyromonas gingivalis (ATCC 33277 and W83) bound to KB gingival epithelial cells were analyzed by SDS-PAGE and immunoblotting. We found that a 51-kDa component was formed in bacteria that adhered to the KB cells, whereas 26- to 29-kDa bands were less intensive, in contrast to the protein profile of free bacteria. P. gingivalis ATCC 33277 incubated with protease-treated KB cells retained the profile of free bacteria. These results demonstrate the specificity of bacterial recognition of eukaryotic membrane components.