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Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia
H Yamada1, T Murakami, T Kaneda
1Department of Hematology, Nagoya National Hospital, Japan. hayabusa@kctv.ne.jp
Background:
Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET).
Methods:
The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis.
Results:
Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients).
Conclusion:
Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.
