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Multiple roles for the yeast SUB2/yUAP56 gene in splicing
D Libri1, N Graziani, C Saguez
1Centre National de la Recherche Scientifique, Center Génétique Moléculaire, 91190 Gif sur Yvette, France. Libri@cgm.cnrs-gif.fr
Genes & Development
|January 13, 2001
Summary
The Schizosaccharomyces pombe SUB2 gene is crucial for spliceosome assembly. This study identifies its role in yeast splicing, revealing its involvement in multiple steps of the process.
Area of Science:
- Molecular Biology
- Genetics
- RNA Splicing
Background:
- The UAP56 gene is essential for prespliceosome assembly in mammals.
- The function of UAP56 orthologs in other organisms is less understood.
Purpose of the Study:
- To isolate and characterize the Schizosaccharomyces pombe ortholog of the UAP56 gene.
- To investigate the role of the SUB2 gene in spliceosome assembly in vivo and in vitro.
Main Methods:
- Heterologous complementation of a combined PRP40HA(3)/nam8Delta defect in budding yeast.
- In vivo and in vitro splicing assays using Sub2-defective yeast extracts.
Main Results:
- The Schizosaccharomyces pombe UAP56 ortholog, SUB2, was isolated.
- Sub2-defective extracts exhibited a stalled intermediate containing U2snRNP, which could be chased into functional spliceosomes.
- Evidence suggests Sub2p involvement in events preceding prespliceosome formation.
Conclusions:
- Sub2p plays a critical role in multiple stages of spliceosome assembly in yeast.
- The findings implicate Sub2p in both early and later steps of the splicing process.