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Updated: Oct 9, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Burkholderia cepacia genomovar III Is a common plant-associated bacterium
J Balandreau1, V Viallard, B Cournoyer
1Ecologie Microbienne, UMR 5557 CNRS-Université Claude Bernard Lyon I, Villeurbanne, France. balandreau@univ-lyon1.fr
Abstract:
A polyphasic taxonomic study involving DNA-DNA hybridization, whole-cell protein electrophoresis, and 16S ribosomal DNA sequence analysis revealed that a group of Burkholderia cepacia-like organisms isolated from the rhizosphere or tissues of maize, wheat, and lupine belong to B. cepacia genomovar III, a genomic species associated with "cepacia syndrome" in cystic fibrosis patients. The present study also revealed considerable protein electrophoretic heterogeneity within this species and demonstrated that the B. cepacia complex consists of two independent phylogenetic lineages.
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