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Nickel-resistance-based minitransposons: new tools for genetic manipulation of environmental bacteria
S Taghavi1, H Delanghe, C Lodewyckx
1Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium.
Applied and Environmental Microbiology
|February 7, 2001
Abstract:
The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha, beta, and gamma subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.