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Sterilization of Mycobacterium tuberculosis Erdman samples by antimicrobial fixation in a biosafety level 3
J R Schwebach1, W R Jacobs, A Casadevall
1Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. schwebac@aecom.yu.edu
Journal of Clinical Microbiology
|February 7, 2001
Abstract:
Incomplete sterilization of Mycobacterium tuberculosis Erdman cultures followed 1 h of incubation in low concentrations of glutaraldehyde (0.5 and 1.0%) or azide. In contrast, 2.5% glutaraldehyde, paraformaldehyde (2 or 4%), Vesphine IIse or 5% formalin sterilized these samples after 1 h. These results suggest caution in removing fixed M. tuberculosis samples from biosafety level 3.