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Immunofluorescence method for detection of Ebola virus immunoglobulin g, using HeLa cells which express recombinant
M Saijo1, M Niikura, S Morikawa
1Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musahimurayama, Tokyo 208-0011, Japan.
Journal of Clinical Microbiology
|February 7, 2001
Abstract:
A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies.