Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
  1. Home
  3. Research Domains

Colonic luminal contents induce cyclooxygenase 2 transcription in human colon carcinoma cells.

B Glinghammar1, J Rafter

  • 1Department of Medical Nutrition, Karolinska Institutet, Novum, Huddinge, Sweden.

Gastroenterology
|February 13, 2001

Related Experiment Videos

View abstract on PubMed

Summary
This summary is machine-generated.

Related Concept Videos

  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Colonic Luminal Contents Induce Cyclooxygenase 2 Transcription In Human Colon Carcinoma Cells.

    • Compounds in the colon can affect cyclooxygenase-2 (COX-2) gene activity, potentially influencing colon cancer development. Dietary factors may play a role in regulating these COX-2-inducing luminal agents.

    Area of Science:

    • Gastroenterology
    • Molecular Biology
    • Cancer Research

    Background:

    • Cyclooxygenase-2 (COX-2) inhibitors show promise in colon cancer prevention.
    • Understanding how colonic luminal contents regulate COX-2 transcription is crucial.

    Purpose of the Study:

    • To investigate the influence of colonic luminal agents on cyclooxygenase-2 (COX-2) gene transcription.
    • To identify specific luminal compounds that modulate COX-2 promoter activity in colon cells.

    Main Methods:

    • Utilized transient transfections with a human COX-2 promoter-luciferase construct in HCT 116 cells.
    • Assessed the effects of pure luminal compounds and fecal water components on luciferase activity.

    Main Results:

    • Deoxycholate, chenodeoxycholate, and butyrate induced COX-2 promoter activity.
    • Lipid extracts from human fecal water also induced COX-2 promoter activity, with inter-individual variability.
    • Induction correlated with activator protein 1 (AP-1) dependent transcription, mediated by protein kinase C and p38 mitogen-activated protein kinase.

    Conclusions:

    • Colonic luminal components can regulate COX-2 transcription.
    • These findings suggest a link between luminal contents, COX-2 regulation, and colon tumor development.
    • Dietary factors likely influence the responsible luminal components.

    Related Experiment Videos