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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

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Published on: October 22, 2012

An incremental increase in the complexity of peptides bound to class II MHC changes the diversity of positively

R Pacholczyk1, P Kraj, L Ignatowicz

  • 1Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|February 13, 2001
PubMed

Positive selection of the normal repertoire of TCRs results from low-avidity interactions with a set of self-peptides bound to the MHC molecules expressed by thymic epithelial cells. The contribution of the individual peptide to positive selection remains a matter of debate. Here, for the first time, we show that two covalent class II MHC-peptide complexes positively select different TCRs expressing a common transgenic TCRbeta-chain and endogenous TCRalpha-chains. Simultaneous expression of both A(b)-peptide complexes changed the diversity of positively selected TCRs, indicating an additive and possibly synergistic effect of various peptides in this process.

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