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Related Experiment Videos

Mouse DREAM/calsenilin/KChIP3: gene structure, coding potential, and expression.

F Spreafico1, J J Barski, C Farina

  • 1Max-Planck-Institute of Neurobiology, Am Klopferspitz 18A, Martinsried, D-82152, Germany.

Molecular and Cellular Neurosciences
|February 13, 2001
PubMed
Summary
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Three calcium-binding proteins (calsenilin, DREAM, KChIP3) originate from a single mouse gene, impacting intracellular signaling and nervous system function. Their expression suggests developmental roles.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Calcium-binding proteins with EF-hands are crucial for intracellular signaling.
  • Three human members of the Neuronal Calcium Sensor (NCS) subgroup have been identified: calsenilin, DREAM, and KChIP3.
  • These proteins have distinct known functions, including interaction with presenilins, transcriptional repression, and potassium channel modulation.

Purpose of the Study:

  • To characterize the mouse full-length cDNA and genomic locus of the gene encoding calsenilin, DREAM, and KChIP3.
  • To investigate the molecular mechanisms generating multiple protein isoforms from this single gene.
  • To determine the expression pattern of these transcripts within the mouse nervous system and during development.

Main Methods:

  • Full-length cDNA and genomic locus sequencing.

Related Experiment Videos

  • Analysis of alternate translation starts and alternative splicing.
  • In situ hybridization, RT-PCR, and Northern blotting for expression analysis.

    • Main Results:

    • The three human NCS proteins are encoded by a single unique gene locus in mice.
    • Alternate translation starts and alternative splicing generate diverse transcripts, including those lacking EF-hand domains.
    • Expression is restricted to the nervous system, correlating with Kv4.2 potassium channel subunit distribution.
    • Transcripts are present in early embryonic stages.

    Conclusions:

    • A single gene locus generates multiple calcium-binding protein isoforms through complex regulatory mechanisms.
    • The nervous system-restricted expression pattern highlights the role of these proteins in neuronal function.
    • Their presence during early development suggests involvement in neurodevelopmental processes.