Periviscerokinins in cockroaches: release, localization, and taxon-specific action on the hyperneural muscle

The periviscerokinins of Periplaneta americana (Pea-PVKs) were the first neuropeptides directly isolated from perisympathetic organs (PSOs), neurohemal swellings of the transverse and median nerves of insects. It has been demonstrated that Pea-PVK-1 release can be induced from the abdominal PSOs of P. americana by in vitro depolarization. A myotropic effect of Pea-PVK-1 on the hyperneural muscle is restricted to blattid cockroaches, whereas proctolin induces contractions of this muscle in all cockroach species investigated. The location and morphology of Pea-PVK-1-like immunoreactive neurons in species of different cockroach taxa are very similar to those previously described for P. americana. Pea-PVK-1-like immunoreactivity is restricted to cells of the abdominal ganglia which constitute a neurohemal system and project via the median nerve to the abdominal PSOs. Despite interspecific differences in the topography of the transverse nerves of the terminal ganglion, Pea-PVK-1-like immunoreactive fibers always innervate transversal nerves VII and VIII of the terminal ganglion. The results suggest that PVKs act as neurohormones throughout the cockroaches, although they may have different effect(s) depending on the species.