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Characterization of the mouse Cblc/Cbl3 gene
F Fiore1, V Ollendorff, D Birnbaum
1Laboratoire d'Oncologie Moléculaire, U.119 Inserm, IFR57, 27 Boulevard Leï Roure, 13009 Marseille, France.
Biochemical and Biophysical Research Communications
|February 13, 2001
Summary
Researchers cloned and characterized the mouse Cblc/Cbl3 gene, identifying a 496-amino acid protein. This gene shows ubiquitous expression in mouse tissues, suggesting broad biological roles.
Area of Science:
- Molecular Biology
- Genetics
- Gene Expression Analysis
Background:
- The Cbl family of proteins plays critical roles in signal transduction and cellular regulation.
- Understanding the specific functions of Cblc/Cbl3 in mice is essential for comparative genomics and disease research.
Purpose of the Study:
- To clone and characterize the mouse Cblc/Cbl3 gene.
- To determine the structural and evolutionary relationship of mouse Cblc/Cbl3 with its human ortholog and other mouse CBL family members.
- To investigate the expression pattern of mouse Cblc mRNA in various embryonic and adult tissues.
Main Methods:
- Gene cloning and sequencing techniques.
- Bioinformatic analysis for protein sequence comparison and domain identification.
- Messenger RNA (mRNA) expression analysis using Northern blotting or RT-PCR (implied).
Main Results:
- The mouse Cblc/Cbl3 gene was successfully cloned and found to comprise 12 exons.
- It encodes a putative protein of 496 amino acids.
- Significant amino acid identity was observed with its human ortholog (67%) and mouse CBL (70%) within conserved domains (SH2 and Ring finger).
- Mouse Cblc mRNA exhibited a ubiquitous expression pattern across embryonic and adult tissues.
Conclusions:
- The mouse Cblc/Cbl3 gene is structurally conserved and shares significant homology with known CBL family members.
- The ubiquitous expression of mouse Cblc suggests its involvement in fundamental cellular processes across various tissues and developmental stages.