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Bone Marrow-derived Macrophage Production
07:06

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Published on: November 22, 2013

Expression of macrophage asialoglycoprotein-binding protein is induced through MAPK classical pathway

R Katsuyama1, A Morioka, S Oka

  • 1Department of Biological Chemistry and CREST (Core Research for Educational Science and Technology) Project, Japan Science and Technology Corporation, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606-8501, Japan.

Biochemical and Biophysical Research Communications
|February 13, 2001
PubMed

Insights

Macrophage asialoglycoprotein-binding protein (M-ASGP-BP) mRNA expression is induced during macrophage differentiation. Protein kinase C (PKC) and mitogen-activated protein kinase (MAPK) pathways mediate this M-ASGP-BP induction.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Macrophage asialoglycoprotein-binding protein (M-ASGP-BP) is a Gal/GalNAc-specific lectin.
  • M-ASGP-BP functions as an endocytosis receptor.
  • M-ASGP-BP mRNA expression is induced during macrophage differentiation from bone marrow cells.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying M-ASGP-BP mRNA induction during macrophage differentiation.
  • To utilize U937 cells as a model system for studying M-ASGP-BP gene regulation.

Main Methods:

  • U937 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce differentiation.
  • The effects of protein kinase C (PKC) inhibitors (calphostin C, staurosporine) and mitogen-activated protein kinase (MAPK) inhibitors (PD98059, SB202190) on M-ASGP-BP mRNA expression were assessed.

Main Results:

  • TPA treatment rapidly induced M-ASGP-BP mRNA expression in U937 cells within 6 hours.
  • PKC inhibitors completely blocked TPA-induced M-ASGP-BP mRNA expression.
  • The MAPK inhibitor PD98059, but not SB202190, inhibited M-ASGP-BP mRNA expression, suggesting involvement of the classical MAPK pathway.

Conclusions:

  • M-ASGP-BP mRNA expression during macrophage differentiation is mediated by the activation of PKC.
  • The classical MAPK pathway is also involved in the induction of M-ASGP-BP mRNA.
  • These findings elucidate key signaling pathways regulating M-ASGP-BP expression in differentiating macrophages.

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