Related Experiment Video
Updated: Oct 9, 2026
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
Development of an ELISA for the diagnosis of Corynebacterium pseudotuberculosis infections in goats
J Kaba1, L Kutschke, G F Gerlach
1Katedra Chorob Zakaznych, Mikrobiologii i Parazytologii, Wydzial Medycyny Weterynaryjnej SGGW, Grochowska 272, 03-849, Warszawa, Poland. kaba@amaltea.sggw.waw.pl
Abstract:
An ELISA was developed for the diagnosis of Corynebacterium pseudotuberculosis infections in goats. A bacterial whole cell extract was used as solid-phase antigen, and serum from a culture-positive animal served as the internal reference standard. The well-to-well and assay-to-assay variations were determined to be 12.7 and 33.0%, respectively. A cut off value was determined by parallel testing of 142 sera (112 ELISA-positive, 30 ELISA-negative) in a Western blot, and the correlation between both tests was highly significant (K=0, 93). In addition, the reliability of the ELISA for the detection of infected herds was proven in a double blind study testing 910 sera from 74 goat herds.
