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Updated: Aug 10, 2026

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 29, 2010
Prolactin activation of IRF-1 transcription involves changes in histone acetylation
1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Abstract:
In response to prolactin (PRL) signaling, transcription of the interferon regulatory factor-1 gene (IRF-1) is rapidly induced during early G(1), declines in mid G(1), and rises again over the G(1)/S transition phase of the cell cycle in Nb2 T cells. Using chromatin immunoprecipitation assays, we show that histone H4 acetylation increases over a 1.7 kb region of the IRF-1 promoter in early G(1) and again at the G(1)/S transition in response to PRL stimulation. These results demonstrate a correlation between histone H4 hyperacetylation at the IRF-1 promoter and biphasic transcription of IRF-1 in response to PRL signaling in vivo.
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