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Related Experiment Videos

Vaccination against ovine footrot using a live bacterial vector to deliver basic protease antigen.

R J Moore1, D J Stewart, K Lund

  • 1CSIRO Division of Animal Health, Animal Health Research Laboratory, Private Bag 1, Parkville, Vic. 3052, Australia. robert.moore@li.csiro.au

FEMS Microbiology Letters
|February 13, 2001
PubMed
Summary
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A novel Corynebacterium pseudotuberculosis vaccine vector expressing Dichelobacter nodosus basic protease elicited an immune response in sheep but did not prevent footrot. However, disease progression was observed to slow down.

Area of Science:

  • Veterinary vaccinology
  • Bacterial genetics
  • Immunology

Background:

  • Corynebacterium pseudotuberculosis strain Toxminus, attenuated by phospholipase D gene deletion, is a candidate live vaccine vector.
  • Dichelobacter nodosus causes ovine footrot, a significant veterinary concern.

Purpose of the Study:

  • To develop a recombinant vaccine vector expressing the Dichelobacter nodosus basic protease gene.
  • To evaluate the efficacy of this recombinant vaccine in sheep.

Main Methods:

  • A recombinant Corynebacterium pseudotuberculosis strain was created by introducing the Dichelobacter nodosus basic protease gene on a high-copy-number plasmid (pEP2).
  • Sheep were vaccinated with the recombinant strain, and immune responses were assessed.
  • Vaccinated sheep were challenged with Dichelobacter nodosus to evaluate protection against footrot.

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Main Results:

  • The recombinant strain successfully secreted the Dichelobacter nodosus basic protease protein.
  • Vaccination elicited a detectable IgG immune response in sheep.
  • Animals vaccinated with the recombinant strain were not fully protected against footrot challenge.
  • A trend towards slowed disease progression was observed in vaccinated sheep.

Conclusions:

  • The recombinant Corynebacterium pseudotuberculosis vector expressing Dichelobacter nodosus basic protease elicits an immune response but does not confer complete protection against ovine footrot.
  • Further research may be warranted to optimize this vaccine vector system for improved efficacy.