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Adult human olfactory stem cells.

F J Roisen1, K M Klueber, C L Lu

  • 1Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, 500 S. Preston St., Louisville, KY 40202, USA. fjrois01@gwise.louisville.edu

Brain Research
|February 13, 2001
PubMed
Summary
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Human olfactory neuroepithelium (ONe) offers a practical source of pluripotent stem cells for research. These readily accessible cells show continuous regeneration and potential for central nervous system (CNS) repair strategies.

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Adult central nervous system (CNS) stem cells are difficult to access for transplantation.
  • Limited availability and histocompatibility issues hinder CNS stem cell use.
  • Olfactory neuroepithelium (ONe) presents an accessible alternative due to its regenerative capacity.

Purpose of the Study:

  • To investigate the potential of human ONe to yield viable pluripotent stem cell populations.
  • To characterize the properties of cells derived from human ONe.
  • To explore the utility of these cells for potential CNS repair.

Main Methods:

  • Human ONe harvested from cadavers (6-18h postmortem).
  • Dissociation, plating, and culture of ONe cells.

Related Experiment Videos

  • Identification of cell populations using lineage-specific markers.
  • Exposure to dibutyryl-cAMP to assess growth modulator effects.
  • Viability assessment using MTT assay.

    • Main Results:

    • Heterogeneous neuronal, glial, and epithelial cell populations identified.
    • 5-10% of cultures yielded rapidly dividing cells forming neurospheres.
    • Neurospheres contained neuronal and glial subpopulations (A2B5, GFAP positive).
    • Dibutyryl-cAMP reduced cell division and increased process formation.
    • Cells maintained viability after >70 passages, irrespective of donor age/sex.

    Conclusions:

    • Human ONe is a viable source for generating neurosphere-forming cells.
    • These cells exhibit neuronal and glial differentiation potential.
    • Ex vivo expansion provides patient-specific cells for evaluation and potential CNS repair.