Cytokine measurement techniques for assessing hypersensitivity

One of the most potentially useful tools in immunotoxicology is the assessment of cytokines, the molecules responsible for regulating a variety of processes including immunity, inflammation, apoptosis, and hematopoiesis. The particular profile of cytokine production may provide important information regarding the nature of an immunotoxic response such as hypersensitivity (i.e. TH(1) vs. TH(2) response). Proper evaluation of the role of cytokines in understanding hypersensitivity reactions requires attention to a multitude of technical details. Some of the details discussed in this review include the source of the sample to be tested (circulating, local, or ex vivo isolated cells), the potential effects of collection, processing, and storage on the results of the assays, potential variables associated with the source material (matrix effects, relevance, inhibitory substances), and factors influencing the choice of assay used (bioassay, immunoassay, molecular biology technique, flow cytometry). Other often-overlooked issues are discussed, including species considerations and quality control issues such as the use of reference standards and the expression of results.