Expression of the estradiol-synthesizing 17beta-hydroxysteroid dehydrogenases type 1 and type 7 in the nonhuman...

The common marmoset monkey (Callithrix jacchus) was used as a primate model for the study of the expression of the estradiol synthesizing enzymes 17beta-hydroxysteroid dehydrogenase type 1 and type 7 (17HSD1 and 17HSD7). The tissue-specific expression of 17HSD1 and 17HSD7 mRNA in Callithrix jacchus and human as shown by Northern Blot analysis revealed strong similarities between the two species. After cloning of the marmoset-specific coding cDNA sequence of 17HSD7 a similarity of 95% to the known human sequences was found. To elucidate the physiological function of 17HSD7 which is thought to be different to that of the well-known 17HSD1, the regulation of 17HSD7 expression in the corpus luteum was investigated. It was shown to be upregulated during the luteal phase of the reproductive cycle and during early pregnancy, when the primate corpus luteum is most active in estradiol synthesis, whereas 17HSD1 was not detectable in this tissue at any time.