Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Combining nucleic acid amplification and detection.

B Schweitzer¹, S Kingsmore

¹Molecular Staging Inc., 66 High Street, Guilford, CT 06437, USA. barrys@molecularstaging.com

Current Opinion in Biotechnology

|February 13, 2001

Summary

Recent molecular amplification advancements include new technologies like rolling circle amplification and Invader, more diagnostic assays, and novel read-out probes such as Scorpions and nanoparticles.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dyspnoea: Pathophysiology and a clinical approach.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2016

Same author

Postoperative pain assessment in children: a pilot study of the usefulness of the analgesia nociception index.

British journal of anaesthesia·2015

Same author

Effects of β2 agonists on post-thoracotomy pain incidence.

European journal of pain (London, England)·2015

Same author

A socio-ecological approach promoting physical activity and limiting sedentary behavior in adolescence showed weight benefits maintained 2.5 years after intervention cessation.

International journal of obesity (2005)·2014

Same author

Growth limitation of planktonic bacteria in a large mesotrophic lake.

Microbial ecology·2013

Same author

Use of dissolved carbohydrates by planktonic bacteria in a mesotrophic lake.

Microbial ecology·2013

Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

Molecular amplification techniques are crucial for detecting and quantifying nucleic acids.
The field continuously seeks more sensitive, specific, and efficient amplification methods.

Purpose of the Study:

To summarize key recent advancements in molecular amplification technologies and applications.
To highlight innovations in amplification methods, diagnostic assays, and detection probes.

Main Methods:

Review of recent literature on molecular amplification.
Focus on validation of new amplification technologies.
Analysis of trends in molecular diagnostic assay development and direct amplification on microarrays.
Examination of novel read-out probe designs.

Main Results:

Initial validation of two novel amplification technologies: rolling circle amplification and Invader.
A significant increase in the number of available molecular diagnostic assays.
Successful achievement of direct amplification on microarrays using strand displacement amplification and rolling circle amplification.
Introduction of two new read-out probes: Scorpions and nanoparticles.

Conclusions:

The past year has seen substantial progress in molecular amplification.
These advances promise to enhance molecular diagnostics and research capabilities.

Related Experiment Videos