Related Experiment Video
Updated: Aug 19, 2026
An Isolated Working Heart System for Large Animal Models
Published on: June 11, 2014
Right ventricular support for off-pump coronary artery bypass grafting studied with bi-ventricular pressure--volume
A L Dekker1, G G Geskes, A A Cramers
1Department of Cardiothoracic Surgery, Cardiovascular Research Institute Maastricht, Academic Hospital Maastricht, P. Debyelaan 25, 6229HX, Maastricht, The Netherlands.
Objectives:
Tilting the heart during off-pump coronary artery bypass grafting (OPCABG) causes a strong decrease in cardiac output. It is hypothesized that this decrease is caused by reduced right ventricular filling and that right ventricular support is thus the best way to restore cardiac output. Simultaneous left and right ventricular pressure-volume loops were used to test this hypothesis.
Methods:
In seven sheep, the heart was tilted with the use of an Octopus device. After unsupported tilting, a novel right ventricular support, the Enabler, was activated at a pulsatile flow of 1.6 l/min. Pressure-volume loops of both ventricles were obtained using conductance catheters, and cardiac output was monitored with an aortic flow probe.
Results:
Tilting reduced cardiac output by 31% (4.4--3.1 l/min, P=0.001) and right ventricular end-diastolic volume by 44% (86--51 ml, P=0.005), while right ventricular end-diastolic pressure did not decrease. Left ventricular systolic pressure was not significantly reduced upon tilting and even increased in two animals. During Enabler right ventricular support, the cardiac output remained 23% lower than pre-tilting values (3.4 vs. 4.4 l/min, P=0.001).
Conclusions:
Restricted right ventricular filling is the primary cause of the strong decrease in cardiac output during tilting. The Enabler right ventricular support can currently not restore cardiac output to pre-tilting values, mainly caused by its limited output and a decrease in right ventricular output upon Enabler activation. Constant monitoring of cardiac output is crucial during (unsupported or supported) tilting as blood pressure alone may not reflect the extent of the reduction in cardiac function.
