Related Experiment Videos
Construction and characterization of a gridded cattle BAC library
J Buitkamp1, S Kollers, G Durstewitz
1Lehrstuhl für Tierzucht, Technische, Universität München, Freising-Weihenstephan, Germany.
Animal Genetics
|February 13, 2001
Summary
A comprehensive bovine bacterial artificial chromosome (BAC) library was created for cattle genomics research. This resource aids in mapping and sequencing the bovine genome, advancing cattle genetic studies.
Area of Science:
- Genomics
- Molecular Biology
- Animal Genetics
Background:
- Bovine genomic research requires advanced tools for large-scale DNA analysis.
- Bacterial Artificial Chromosome (BAC) libraries are crucial for constructing physical maps and sequencing genomes.
Purpose of the Study:
- To construct and characterize a large-insert BAC library from Holstein-Friesian cattle.
- To facilitate further genetic and genomic studies in cattle.
Main Methods:
- Genomic DNA from Holstein-Friesian leukocytes was size-fractionated and ligated into a pBACe3.6 vector.
- Pulsed-field gel electrophoresis (PFGE) was used to determine insert sizes.
- Fluorescence in situ hybridization (FISH) mapped BAC clones to cattle chromosomes.
Main Results:
- A library of approximately 83,000 BAC clones was generated with an average insert size of 104 kb.
- 5.5% of clones lacked inserts.
- 34 BAC clones were successfully mapped to cattle chromosomes, with some indicating repetitive elements.
Conclusions:
- The constructed BAC library is a valuable resource for bovine genome research.
- The library provides a foundation for developing sequence-tagged sites and physical mapping.
- Availability through the German Human Genome Project facilitates broader research access.