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Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide
L Karim1, W Coppieters, L Grobet
1Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Belgium.
Animal Genetics
|February 13, 2001
Abstract:
We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.