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Updated: Aug 12, 2026
A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
Published on: March 8, 2014
Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients
A Bellahcène1, I Van Riet, C de Greef
1Metastasis Research Laboratory, University of Liège, Belgium. a.bellahcene@ulg.ac.be
Abstract:
Bone sialoprotein (BSP) is a glycoprotein essentially found in mineralizing connective tissues. We have recently demonstrated that BSP is ectopically expressed by carcinomas that metastasize to bone with high frequency. Multiple myeloma (MM) is characterized by the localization of tumour plasma cells in the bone marrow. In this study, BSP expression was evaluated in human myeloma cell lines and in bone marrow aspirates and one ascites fluid from MM patients. BSP was detectable in conditioned media of MM cell lines. Using FACS analysis and in situ hybridization, we demonstrated that tumour cells from all MM patients and cell lines analysed express BSP at both the protein and the mRNA level.
Insights
Bone sialoprotein (BSP) is expressed by multiple myeloma (MM) cells. This study confirms BSP protein and mRNA expression in human myeloma cell lines and patient samples, indicating its role in MM bone marrow localization.
Area of Science:
- Biochemistry
- Oncology
- Cell Biology
Background:
- Bone sialoprotein (BSP) is a key glycoprotein in mineralizing connective tissues.
- Aberrant BSP expression is linked to carcinoma metastasis to bone.
- Multiple myeloma (MM) is characterized by tumor plasma cell infiltration of the bone marrow.
Purpose of the Study:
- To investigate BSP expression in multiple myeloma (MM).
- To determine if MM cells express BSP at the protein and mRNA levels.
Main Methods:
- Analysis of BSP in conditioned media from human myeloma cell lines.
- Flow cytometry (FACS) analysis of BSP protein expression.
- In situ hybridization for BSP mRNA detection in patient samples.
Main Results:
- BSP was detected in the conditioned media of MM cell lines.
- Tumor cells from all analyzed MM patients and cell lines expressed BSP protein.
- BSP mRNA was also detected in tumor cells from MM patients and cell lines.
Conclusions:
- BSP is expressed by multiple myeloma cells.
- BSP expression in MM cells may contribute to their bone marrow localization and associated pathology.
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