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Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability
1Fish Health Unit, Department of Primary Industries, Water & Environment, PO Box 46, Kings Meadows, Launceton, Tasmania 7249, Australia. jeremy.carson@dpiwe.tas.gov.au
Aims:
To develop miniaturized tests for the phenotypic identification of motile Aeromonas species using an improved probability matrix.
Methods And Results:
Conventional tests were miniaturized for use in 96-well plates, and their performance assessed using 60 aeromonads comprising type and reference strains as well as clinical, fish and water isolates. A revised probability matrix for Aeromonas hybridization groups 1-14, including A. allosaccharophila, A. bestiarum, A. encheleia and A. popoffii, was developed. Using 26 tests, all the reference strains were correctly identified with the revised probability matrix, and 80% of the isolates were correctly identified at a Willcox probability level of 95%.
Conclusion:
The compact test format, coupled with a robust identification matrix, provides a convenient basis for identifying motile aeromonads.
Significance And Impact Of The Study:
The identification system for identifying aeromonads will be of use to medical and veterinary laboratories undertaking disease diagnosis.