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Programmed cell death in human acute cutaneous wounds.

M Edwards1, D Jones

  • 1University of Wales Institute Cardiff, School of Applied Sciences, Llandaff Campus, Cardiff, Wales, UK. medwards@uwic.ac.uk

Journal of Cutaneous Pathology
|February 13, 2001
PubMed
Summary

Programmed cell death, or apoptosis, clears inflammatory cells from healing skin wounds. Apoptosis in early healing (7-day) wounds does not correlate with later stages (42-day) of wound repair.

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Area of Science:

  • Dermatology
  • Cell Biology
  • Wound Healing Research

Background:

  • Effective resolution of inflammation is crucial for proper cutaneous wound healing.
  • Delayed inflammatory resolution can impede wound maturation and closure.

Purpose of the Study:

  • To investigate the role of apoptosis in the resolution of the inflammatory infiltrate during human cutaneous wound healing.
  • To quantify the presence and distribution of apoptotic cells at different stages of wound healing.

Main Methods:

  • Histological examination of tissue sections from human cutaneous wounds.
  • In situ TdT-mediated dUTP end-labeling (TUNEL assay) to identify DNA fragmentation in apoptotic cells.
  • Spearman's rank correlation coefficient to assess relationships between apoptosis levels at different time points.

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Main Results:

  • Apoptotic cells were identified in the dermal inflammatory infiltrate and granulation tissue of 7-day and 42-day healing wounds.
  • The percentage of apoptotic cells within the dermal inflammatory infiltrate increased significantly from 30.5% at 7 days to 60.7% at 42 days.
  • No correlation was found between the extent of apoptosis in 7-day wounds and apoptosis in 42-day wounds.

Conclusions:

  • Programmed cell death (apoptosis) is a key mechanism for eliminating inflammatory cells during the healing of acute human cutaneous wounds.
  • Apoptosis contributes to the resolution of the dermal inflammatory infiltrate, facilitating proper wound repair.