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Related Experiment Videos

Protein-fatty acid complex for enhanced loading and stability within biodegradable nanoparticles.

H S Yoo1, H K Choi, T G Park

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.

Journal of Pharmaceutical Sciences
|February 13, 2001
PubMed
Summary

Researchers developed a novel method to create stable, biodegradable nanoparticles encapsulating lysozyme (a protein) using sodium oleate. These protein-loaded nanoparticles show promise for oral delivery and mucosal vaccination.

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Area of Science:

  • Biomaterials Science
  • Protein Chemistry
  • Nanotechnology

Background:

  • Lysozyme is a protein with therapeutic potential.
  • Developing stable formulations for oral protein delivery remains a challenge.
  • Hydrophobic modification can enhance protein stability.

Purpose of the Study:

  • To hydrophobically modify lysozyme using sodium oleate.
  • To formulate the modified lysozyme into biodegradable nanoparticles.
  • To evaluate the stability and encapsulation efficiency of the nanoparticles for potential oral delivery.

Main Methods:

  • Ion-pairing mechanism utilizing sodium oleate and lysozyme.
  • pH-dependent complex formation.
  • Spontaneous emulsion and solvent diffusion for nanoparticle formulation.

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  • Characterization of nanoparticle size, distribution, and encapsulation efficiency.

    • Main Results:

    • Lysozyme-oleate complex formation was confirmed and shown to be pH-dependent.
    • The complex exhibited enhanced conformational stability at elevated temperatures in organic solvents.
    • Biodegradable nanoparticles with near 100% encapsulation efficiency and <100 nm diameter were successfully prepared.
    • High loading capacity (up to 18.6% w/w) was achieved, with increased particle size.

    Conclusions:

    • A novel method for creating stable, biodegradable protein-loaded nanoparticles was demonstrated.
    • The developed nanoparticles offer high encapsulation efficiency and stability.
    • This formulation holds potential for oral protein delivery, including mucosal vaccination applications.