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Maize myosins: diversity, localization, and function.

L Liu1, J Zhou, T C Pesacreta

  • 1Biological Laboratories, Harvard University, Cambridge, Massachusetts, USA.

Cell Motility and the Cytoskeleton
|February 13, 2001
PubMed
Summary
This summary is machine-generated.

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This study identified five monocot myosin genes in maize, with ZMM1 and ZMM2 belonging to class XI and ZMM3 to class VIII. Myosin XI is abundant in various tissues and concentrated in mitochondria and membranes.

Area of Science:

  • Molecular Biology
  • Plant Genetics

Background:

  • Myosins are essential motor proteins involved in various cellular processes.
  • Understanding myosin gene families in monocots like maize is crucial for plant development research.

Purpose of the Study:

  • To analyze the myosin gene family in maize (Zea mays L.).
  • To characterize the expression patterns and localization of specific myosin genes.

Main Methods:

  • Gene sequencing and analysis
  • Phylogenetic analysis
  • Immunofluorescence microscopy
  • Western blot analysis

Main Results:

  • At least five monocot myosin genes were identified in maize, including ZMM1 (class XI), ZMM2 (class XI), and ZMM3 (class VIII).

Related Experiment Videos

  • ZMM1 showed abundant expression in leaves, roots, coleoptiles, and stems. ZMM3 was expressed in similar tissues but poorly in pollen. ZMM2 was predominantly found in seeds.
  • Myosin XI was localized in the cytoplasm of root tip cells, with higher concentrations in differentiating epidermal cells and near the cytokinetic apparatus in dividing cells. It was concentrated in mitochondria and low-density membranes.

    • Conclusions:

    • The myosin gene families, classes VIII and XI, likely originated before angiosperms.
    • Myosin XI plays a significant role in maize root cell structure and function, particularly in differentiating and dividing cells.