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Related Experiment Videos

Src family kinase function during early Xenopus development.

D C Weinstein1, A A Hemmati-Brivanlou

  • 1Department of Molecular Vertebrate Embryology, The Rockefeller University, New York, NY, USA. weinsd01@doc.mssm.edu

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|February 13, 2001
PubMed
Summary

Fibroblast growth factor (FGF) signaling in development relies on Src-related kinases like Laloo. These kinases, alongside SH-PTP2 phosphatase and bone morphogenetic protein (BMP) pathways, are crucial for mesoderm formation in vertebrates.

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Area of Science:

  • Developmental Biology
  • Molecular Signaling
  • Cell Biology

Background:

  • Fibroblast growth factor (FGF) signaling is critical for embryonic development.
  • Src family kinases are known to be involved in various cellular processes.
  • Laloo, a Src family kinase, functions downstream of the FGF receptor in mesoderm formation.

Purpose of the Study:

  • To investigate the role of Src family kinases as general transducers of FGF signaling.
  • To characterize the involvement of SH-PTP2 phosphatase and bone morphogenetic protein (BMP) signaling in Laloo-mediated mesoderm induction.
  • To determine if mesoderm-inducing activity is unique to Laloo or shared among Src family kinases.

Main Methods:

  • Expression analysis of Laloo to assess its function in distinct contexts.

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  • Biochemical characterization of SH-PTP2 phosphatase activity.
  • Investigation of bone morphogenetic protein (BMP) pathway signaling.
  • Functional assays to evaluate mesoderm induction by Src family kinases.

    • Main Results:

    • Laloo expression was shown to mimic FGF function in a novel context, supporting its role as a general FGF signaling transducer.
    • SH-PTP2 phosphatase and BMP pathway signaling were identified as key components in Laloo-induced mesoderm formation.
    • Mesoderm-inducing activity was observed not only in Laloo but also in other Src family kinases, suggesting functional overlap.

    Conclusions:

    • Src-related kinases, including Laloo, are essential and general transducers of FGF signaling during vertebrate development.
    • SH-PTP2 phosphatase and BMP signaling pathways cooperate with Src kinases in mesoderm induction.
    • Members of the Src family kinase gene family exhibit functional redundancy in mediating mesoderm formation.