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Glucocorticoid inhibition of protein synthesis in vivo and in vitro

Y S Kim, Y Kim

    The Journal of Biological Chemistry
    |March 25, 1975
    PubMed

    Insights

    Pharmacological glucocorticoids initially inhibit liver protein synthesis in rats, with repeated doses leading to sustained suppression. This effect is localized to the liver's soluble fraction.

    Area of Science:

    • Biochemistry
    • Endocrinology
    • Molecular Biology

    Background:

    • Glucocorticoids are key regulators of metabolic processes.
    • Their effects on protein synthesis, particularly in the liver, require detailed investigation.

    Purpose of the Study:

    • To investigate the impact of pharmacological glucocorticoid doses on hepatic protein synthesis in vivo and in vitro.
    • To determine the temporal effects of single and multiple glucocorticoid administrations.

    Main Methods:

    • In vivo pulse-labeling experiments in young adult rats.
    • Administration of single and multiple doses of glucocorticoids.
    • Analysis of protein synthesis using cell-free liver systems.

    Main Results:

    • A single glucocorticoid dose initially inhibited, then stimulated liver protein synthesis.
    • Multiple doses resulted in pronounced, sustained inhibition without subsequent stimulation.
    • The inhibitory effect was transferable via the soluble fraction of liver cells.

    Conclusions:

    • Glucocorticoids exert complex, dose-dependent effects on hepatic protein synthesis.
    • Sustained exposure leads to persistent inhibition, suggesting a critical role for the liver's soluble components.

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