Related Experiment Video

Updated: Aug 21, 2026

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

Interactions between adenovirus proteins and the p53 pathway: the development of ONYX-015

F McCormick1

  • 1Cancer Research Institute, UCSF Cancer Comprehensive Cancer Center, San Francisco, CA 94115, USA. mccormick@cc.ucsf.edu

Seminars in Cancer Biology
|February 15, 2001
PubMed

Insights

dl1520 (ONYX-015), an adenovirus mutant, shows promise in cancer therapy. It replicates selectively in tumor cells, despite not correlating with p53 status due to additional E1B 55K functions and alternative p53 loss mechanisms.

Area of Science:

  • Oncolytic virology
  • Molecular oncology

Background:

  • dl1520 (ONYX-015) is an adenovirus mutant engineered to lack the E1b 55K gene.
  • This genetic modification prevents dl1520 from neutralizing the tumor suppressor protein p53.

Purpose of the Study:

  • To investigate the replication characteristics of dl1520 (ONYX-015) in cancer cells.
  • To understand the factors influencing selective viral replication in tumors.

Main Methods:

  • Analysis of dl1520 replication in cancer cell lines with varying p53 statuses.
  • Evaluation of the role of E1B 55K gene functions beyond p53 neutralization.
  • Assessment of alternative mechanisms of p53 pathway inactivation, such as p14ARF loss.

Main Results:

  • Replication of dl1520 did not correlate with p53 status in cancer cells.
  • Additional functions of E1B 55K influence viral replication in a context-dependent manner.
  • Loss of p53 function through alternative pathways, like p14ARF loss, affects dl1520 replication.
  • dl1520 is attenuated in normal cells, where it induces p53.

Conclusions:

  • The selective replication of dl1520 in cancer cells is complex and not solely dependent on p53 status.
  • E1B 55K's multifaceted roles and alternative p53 inactivation pathways contribute to variable viral efficacy.
  • ONYX-015 remains a promising therapeutic candidate undergoing clinical trials for cancer treatment.

More Related Videos

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting08:14

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Published on: October 26, 2018

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...