Related Experiment Videos
Glycosylphosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha
H Okuyama1, S Hagiwara, T Okamoto
1Graduate School Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan. hoku@ees.hokudai.ac.jp
Biochemical Society Transactions
|February 15, 2001
Abstract:
A suspension culture of the moss Marchantia polymorpha was incubated with either [3H]ethanolamine or [3H]myristic acid, precursors of a glycosylphosphatidylinositol (GPI) anchor. By both of these a secretory protein of 47 kDa was consistently labelled. Amino acid composition analysis of the chromatographically purified 47-kDa protein exhibited the occurrence of ethanolamine and glucosamine at approximate molar ratios to protein of 1 and 4, respectively. Thus the secretory 47-kDa protein of M. polymorpha was concluded to be a GPI-anchored protein.