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Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
Published on: November 30, 2013
Mutagenesis of the borage Delta(6) fatty acid desaturase
O Sayanova1, F Beaudoin, B Libisch
1IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK. olga.sayanova@bbsrc.ac.uk
Abstract:
The consensus sequence of the third histidine box of a range of Delta(5), Delta(6), Delta(8) and sphingolipid desaturases differs from that of the membrane-bound non-fusion Delta(12) and Delta(15) desaturases in the presence of glutamine instead of histidine. We have used site-directed mutagenesis to determine the importance of glutamine and other residues of the third histidine box and created a chimaeric enzyme to determine the ability of the Cyt b(5) fusion domain from the plant sphingolipid desaturase to substitute for the endogenous domain of the Delta(6) desaturase.
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