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Related Experiment Videos

Lipase activity in germinating sunflower seedlings.

S Bahri1

  • 1Faculté des Sciences de Tunis, Département de Biologie, Campus Universitaire 1060, Tunis, Tunisia. sellama.bahri@fst.znu.tn

Biochemical Society Transactions
|February 15, 2001
PubMed
Summary

Researchers identified adenosine triphosphate (ATP) as an activator of lipase activity in sunflower seedlings. CHAPS detergent significantly enhanced lipolytic activity, suggesting enzyme complexity and providing insights into lipase

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Area of Science:

  • Plant biochemistry
  • Enzymology
  • Lipid metabolism

Background:

  • Lipase activity is crucial for lipid mobilization during seed germination.
  • Understanding lipase regulation and localization is key to elucidating plant metabolic processes.

Purpose of the Study:

  • To identify activators of lipolytic activity in germinating sunflower seedlings.
  • To investigate the effect of detergents on lipase solubilization and activity.
  • To determine the subcellular localization of lipase.

Main Methods:

  • Assay of lipolytic activity in the presence of adenosine triphosphate (ATP).
  • Solubilization of lipid-body lipase using Tween 80 and CHAPS detergents.
  • Analysis of lipase activity in subcellular fractions (10000 g pellet).

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Main Results:

  • Adenosine triphosphate (ATP) increased lipase activity two-fold.
  • CHAPS detergent enhanced lipolytic activity ten-fold, indicating enzyme complexity.
  • Co-incubation of pellet extract with oil-body fraction resulted in a four-fold increase in lipolytic activity.

Conclusions:

  • ATP acts as a potential activator of sunflower seedling lipase.
  • CHAPS detergent effectively solubilizes and potentially modulates lipase activity.
  • Lipase is likely present in the 10000 g pellet fraction, suggesting specific subcellular localization and involvement in intracellular transport pathways.