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Lipoxygenases in early Brassica napus germination
1Department of Physiology, Carlsberg Laboratory, Gamle Carlsbergvej 10, DK-2500 Valby, Denmark.
Biochemical Society Transactions
|February 15, 2001
Abstract:
We have identified transcripts of two lipoxygenase (LOXs) from 3-day-germinated etiolated cotyledons. One encodes a soluble and the other encodes a plastid LOX. They are 2520 bp and 2676 bp in size, respectively. Antibodies raised against the C-terminal 500 amino acids of the plastid LOX recognize a 90 kDa LOX in 5-day-old etiolated and green cotyledons. In mature leaves a LOX of 95 kDa is detected. LOX activity and protein was mainly located in the cytosol.