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Stimulation of IL-8 release from epithelial cells by gas cooker PM(10): a pilot study
C A Dick1, M Dennekamp, S Howarth
1Napier University, School of Life Sciences, 10 Colinton Road, Edinburgh, EH10 5DT, Scotland, UK. c.dick@napier.ac.uk
Objective:
To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 microm (PM10), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2).
Method:
Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system.
Results:
Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8.
Conclusion:
PM10 generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.