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The complete nucleotide sequence of porcine adenovirus serotype 5
Miklós Nagy1, Éva Nagy2, Tamás Tuboly1
1Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary1.
Abstract:
The complete nucleotide sequence of porcine adenovirus serotype 5 (PAdV-5) has been determined and the putative genomic map was constructed. The size of the genome was found to be 32621 nucleotides. Twenty-eight putative ORFs were identified by their homology to other adenovirus or other virus and eukaryotic genes. Several special protein sequence motifs were identified by their homology to similar protein motifs. The putative promoter regions, polyadenylation and splice sites were predicted and the early and late transcription units were determined. Based on sequence analysis and RNA secondary structure prediction, sequences for virus-associated RNA could not be recognized. Phylogenetic analysis showed that PAdV-5 was more closely related to certain bovine adenoviruses than to other porcine adenoviruses.