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Related Experiment Videos

Distinct signals generate repeating striped pattern in the embryonic parasegment.

V Hatini1, S DiNardo

  • 1Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. hatiniv@mail.med.upenn.edu

Molecular Cell
|February 15, 2001
PubMed
Summary
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Repeating striped patterns in Drosophila are formed by distinct signaling events. Hedgehog (Hh) and Wingless (Wg) ligands first establish territories, then Hh, Spitz (Spi), and Wg induce Stripe (Sr) expression in adjacent areas.

Area of Science:

  • Developmental biology
  • Cellular signaling
  • Pattern formation

Background:

  • Understanding how repeating cellular patterns form is a fundamental question in biology.
  • The precise mechanisms governing the spatial organization of gene expression, particularly in striped patterns, remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying the formation of repeating Stripe (Sr) expression patterns within the parasegment (PS) of Drosophila.
  • To investigate the roles of specific signaling ligands in pattern generation and the regulation of gene expression width.

Main Methods:

  • Analysis of Stripe (Sr) expression patterns in Drosophila parasegments.
  • Investigating the inductive roles of Hedgehog (Hh), Wingless (Wg), and Spitz (Spi) signaling pathways.

Related Experiment Videos

  • Utilizing enhancer trap lines to study gene regulatory elements within the sr gene.

    • Main Results:

    • The repeating striped pattern of Sr expression is established through a two-step process involving territorial subdivision and subsequent induction.
    • Hedgehog (Hh), Wingless (Wg), and Spitz (Spi) ligands, emanating from specific territories, induce Sr expression in adjacent cellular fields.
    • Signaling strength directly influences the width of Sr expression domains.
    • Enhancer trap analysis revealed separable control elements in the sr gene responding to Spi and Wg, but not Hh.

    Conclusions:

    • Each component of the repeating striped pattern is initiated by a distinct inductive signaling event.
    • The sr gene possesses separable regulatory elements that respond differentially to specific signaling pathways.
    • This study provides a detailed model for how complex cellular patterns are assembled through sequential signaling events.